2026-05-01T16:12:53Z https://yabu.me Pete Carlton https://yabu.me/npub1lzssdx00cq8ahx8ga8atlhzfamxde6zelva02c3s5r7924du29dsslxnh2 https://fediscience.org/system/accounts/avatars/109/293/347/316/588/978/original/0df8c869788d97fd.jpg https://fediscience.org/system/accounts/avatars/109/293/347/316/588/978/original/0df8c869788d97fd.jpg https://yabu.me/nevent1qqst25xesu6hkxmmn7xra2gyn6w4g4qp2p26nnsmhx6gjc3422xy4wszyru2zp5ealqqlkucar5l407uf8hveh8gt8an4atzxzs0c424h3g4k5nemqv 2-photon microscopy works by the simultaneous absorption of 2 far-red/infrared quanta, right? Is there any practical limit to N-photon microscopy — that is, a cell could survive a huge amount of long-wavelength EM waves focused on one spot, and by absorbing e.g. 5? 10? quanta at the same time a fluorophore could be activated? My guess is no, anything more than a few photons would involve lethal levels of infrared etc. heating up the cell. 2024-12-29T04:43:19Z https://yabu.me/nevent1qqsf225fqq3jzeazdgajj70z3wm7ek39s8vw4ref664t5rycj58c40qzyru2zp5ealqqlkucar5l407uf8hveh8gt8an4atzxzs0c424h3g4ks675vj In reply to <a href='/nevent1qqsp9967tty9jhh7ekqrrl4pvxse7vg8phgm0g57u2rak75gl40w2psrxd4kh'>nevent1q…d4kh</a><br/>_________________________<br/><br/>Yep, that&#39;s how I learned it! from John Petruska, @ USC 1992<br/><br/>Also we got:<br/>Q = Q-tamine (rhymes with glutamine)<br/>Y = Y-rosine (rhymes with Tyrosine)<br/>D,E = same alphabetical order as Asp, Glu, and this order corresponds to size order<br/><br/>it&#39;s all so perfect 2024-11-01T01:13:21Z